DROZDOWSKA, L / THANGSTAD, OP / BEISVAAG, T / EVJEN, K / BONES, A / IVERSEN, TH

Source
     ISRAEL JOURNAL OF BOTANY
     Vol. 41, No. 4-6, 1992, page 213-223
Abstract
     The occurrence and distribution of myrosin cells and myrosinases have been followed using immunochemical techniques on
     samples taken from pistils before and after pollination, and seeds harvested at three different stages of maturity. Two
     Brassica napus cultivars (Jet Neuf and Bolko) with a high and a low glucosinolate seed content, respectively, were used.
     Two methods recently developed in our laboratory were applied for detection of the enzyme using light microscopy (LM)
     and transmission electron microscopy (TEM). In the first case, paraffin-embedded sections were sequentially incubated
     with a monoclonal anti-myrosinase antibody and with peroxidase- and fluorescein-isothiocyanate-conjugated secondary
     antibodies. For the TEM localization a polyclonal antibody raised in rabbit against a highly purified myrosinase from white
     mustard was used. Myrosinase activity was detected in the early stages during embryogenesis in both cultivars but was
     higher in the high glucosinolate cultivar. In the early stages of development, typical embryonic myrosinase cells could not be
     detected. Later they appear in cotyledons, hypocotyls, and radicles. A typical, specific, and positive LM localization of
     myrosinase in myrosin cells could be detected both in radicles and the cotyledons. In the radicle the positively identified cells
     are located in the second outermost cell layer in the peripheral cortex; in the cotyledon positively identified myrosin cells
     were found scattered around in the tissue. The positive TEM localization demonstrated gold particles uniformly distributed
     over the matrix in the myrosin grains.
Type
     Article
Journal cat.
     · plant sciences
ISI KeyWords Plus®
     brassica-napus l , localization , plants
ISSN / ISBN
     0021-213X